FastQCFastQC Report
Mon 27 Feb 2023
SRR3128594.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128594.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2813021
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234520.8336944516233615No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC45910.16320532267622603No Hit
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG42550.15126086865330904No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC38560.1370768295010951No Hit
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG35300.1254878651812411No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81350.083.242181
CGTAGGG8100.074.862782
AGGGATG46950.073.5759355
GAATAGG20350.073.0740051
TAAGGGA26950.073.070033
ACGGGTA4650.072.771884
TAGGGCA16050.072.0350954
AGGGTAC14600.070.175855
ATAGGGC18250.070.047093
GAGGGAT46200.069.887394
CGAAGGG26200.069.792632
ATAGGGA33250.069.6853
ACGGGAT10800.068.757074
AGGGAAT40400.068.752775
AGTAGGG46500.068.741232
CGGGTAT3650.068.244415
TAGCGGG17600.068.106532
AGGGCAT25050.067.9185
AAGGGCA43200.067.777934
TAGAGGG47850.067.194762
Produced by FastQC (version 0.11.5)