FastQCFastQC Report
Mon 27 Feb 2023
SRR3128593.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128593.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1883738
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164760.8746439260661515No Hit
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG34670.184048949482359TruSeq Adapter, Index 16 (100% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG32180.17083055074537967TruSeq Adapter, Index 16 (100% over 22bp)
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC30460.1616997692885104TruSeq Adapter, Index 16 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT54550.082.723471
TAGGGTA6800.076.023284
CGTAGGG5250.075.231872
GTAGGGC9150.073.4476243
ATAGGGA20400.073.028423
ATAGCGG4100.072.303881
TAGGGCG3300.071.206374
AGTAGGG30200.071.152662
ACGGGTA2400.070.494314
TAGGGCA11150.069.9674454
TATAGGG16400.069.6697542
AGGGCAT17300.067.641935
CGAAGGG16650.067.494062
AGGGTAA10250.067.399445
GTAGGGT8300.067.3801043
AGGGATG28750.066.693755
TAAGGGA15350.066.437643
AAGGGCA30850.066.4192664
TAAGCGG3900.066.359491
AAGGGAT31850.066.2521064
Produced by FastQC (version 0.11.5)