FastQCFastQC Report
Mon 27 Feb 2023
SRR3128592.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128592.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1768012
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145350.8221098046845836No Hit
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG39520.22352789460705016TruSeq Adapter, Index 16 (100% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG35690.20186514571168068TruSeq Adapter, Index 16 (100% over 22bp)
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC34510.1951909828666321TruSeq Adapter, Index 16 (95% over 24bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTACCCTC23350.13206923934905418No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT49350.082.785241
ACGGGTA1650.079.7534
TAGGGCG3000.076.762264
ATAGCGG3600.074.437651
ATAGGGA17700.073.8149643
TAGGGTA6600.073.344274
GACCGAT1100.072.6301358
CGTAGGG5650.072.365472
ATAGGGC9950.071.794873
AGTAAGG11800.070.9183041
AGGGCAT13550.070.756075
AGTAGGG30350.070.610092
TAAGGGA14350.070.414073
GTAGGGC8200.069.349673
ACGGGAT6850.069.295294
AGGGATG27900.069.064135
TAGGGCA9150.068.8266454
TAAGCGG3650.068.265821
CCGTACA11600.067.65993
AGGGTAT10500.067.586595
Produced by FastQC (version 0.11.5)