FastQCFastQC Report
Mon 27 Feb 2023
SRR3128591.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128591.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1976295
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181270.9172213662433999No Hit
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG42030.2126706792255205TruSeq Adapter, Index 16 (100% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG38600.19531497069010445TruSeq Adapter, Index 16 (100% over 22bp)
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC37710.19081159442289738TruSeq Adapter, Index 16 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62050.080.831441
TAGGGTA7400.077.4835054
CGTAGGG6350.075.504622
TAAGCGG4550.075.487731
ATAGGGA19250.073.976383
ATAGCGG3350.073.033721
AGTAGGG32050.072.0112842
TATAGGG15600.070.80932
ACGGGAT6200.070.4972844
GTAGGGC9700.070.255033
ACGGGTA2100.069.378284
AGAGGGC27900.068.896993
ATAGGGC11500.068.658233
AGGGCAT17050.068.636665
ATGCGGG13000.067.976932
AGGGATG31400.067.803125
TAGGGCG3200.067.559894
AGGGTAA11850.067.423565
CGAGGGA12550.067.033283
ATAAGGG24950.066.127732
Produced by FastQC (version 0.11.5)