FastQCFastQC Report
Mon 27 Feb 2023
SRR3128590.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128590.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2514424
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT384751.5301715223844505No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC26400.10499422531760752No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG25570.10169327050648577No Hit
CGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCT25490.10137510618734151No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT127300.085.944011
TAGGGCA12300.073.360814
ACGGGTA3050.072.421254
CGTAGGG7350.072.292352
AGGGATG32750.071.033295
ATAGGGC15700.070.944163
GTAGGGC11650.070.1926043
AGTAGGG43100.067.5327762
TATAGGG19950.067.17432
AAGAGGG88100.066.6634452
ATAAGGG32150.066.254972
ATAGAGG17350.066.177661
ACGGGAT7350.065.859424
TAGAGGG45100.065.7891162
AAGGGCA36400.065.072454
ATAGCGG5500.065.023741
ATGCGGG12750.064.908822
AGAGGGC37250.064.8492363
GAGGGAT32800.064.620554
ATAGGGA23650.064.384583
Produced by FastQC (version 0.11.5)