FastQCFastQC Report
Mon 27 Feb 2023
SRR3128589.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128589.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2423528
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360591.487872225944986No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC28840.11900006932042873No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG27140.11198550212747696No Hit
CGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCT25740.1062087997332814No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT123400.085.524151
ACGGGTA2600.074.1101764
ATAGGGC14350.073.689723
TAGGGCA13100.072.826944
GTAGGGC10400.072.756013
AGGGATG33550.069.340825
TATAGGG17650.068.9654162
AGTAGGG38050.067.810042
GGTAAGG14600.067.6468351
ATAGAGG18550.067.440191
ATAGGGA21450.067.2647253
CGTAGGG7850.067.0542
AGATAGG13500.066.888051
TAGACGG3450.066.797171
AAGAGGG86650.065.899792
AGAGGGC35900.065.587283
GTAAGGG34750.064.917652
ATAAGGG30950.064.840082
TAAGGGA18650.064.763543
TAGCGGG13000.063.9891322
Produced by FastQC (version 0.11.5)