FastQCFastQC Report
Mon 27 Feb 2023
SRR3128588.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128588.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2762876
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT441111.596560974868217No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG30300.10966833111583726No Hit
CGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCT29470.1066642151149744No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC28730.10398584663227739No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT148600.086.2131651
TAGGGCA13850.074.993454
CGTAGGG8350.072.059422
ATAGGGC16700.071.20083
ATAGGGA24850.069.598883
ATAGCGG6850.069.370621
ACGGGTA2650.069.167094
ACGGGAT8200.068.777794
AGTAGGG45250.068.563442
TAGAGGG49900.068.485892
AGAGGGC42000.068.371133
GTAGGGC11650.068.177583
ATAGAGG20650.068.123341
TAGACGG3900.067.5566561
AGATAGG18250.067.285641
TAGCGGG15000.067.064052
AGGGATG37200.066.707065
AAGAGGG97400.066.022872
GATAGGG41450.065.549682
GTAGGGA21450.065.512583
Produced by FastQC (version 0.11.5)