FastQCFastQC Report
Mon 27 Feb 2023
SRR3128587.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128587.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences768608
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88871.1562460968400017No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC82341.0712873142095842No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTCCCATGG38490.5007754277863358No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG34210.44509034514342816No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC31190.40579853449352593No Hit
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG27890.3628637745118448No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCTCC18060.23497023189974603No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA14730.1916451559182314No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATG13120.17069819726050212No Hit
TAGACCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCT12910.16796598526166784No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC12700.1652337732628336No Hit
CGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT10850.14116428660643657No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA10020.13036554394437735No Hit
ACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG9130.11878616928265125No Hit
AAAGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTT8890.11566364128398351No Hit
AGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT8460.11006911195303717No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGAC353.583409E-1093.997288
CACGGGT201.8377728E-593.997263
GTACGCA201.8377728E-593.997268
ACGGGTA1200.090.080724
CGTTTTT36800.087.8421551
ATAGCGG2200.087.691041
CGTAGGG2000.086.981422
TAGCGGG4900.086.357732
GCTACGA15000.085.537514
AGGGATG22000.085.452065
TCCGCTA15350.085.2178341
AGGGAAT17000.084.8745
CGCTACG15250.084.443443
AGCGGGA7000.083.926133
CTACGAC15450.083.046135
AGGGTAC3700.082.565175
GCGTAGG1600.082.3440251
GGATGGC17450.082.146617
CGGGAAT3150.082.061115
ACGACCA15250.081.977947
Produced by FastQC (version 0.11.5)