FastQCFastQC Report
Mon 27 Feb 2023
SRR3128586.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128586.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences751111
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81701.0877220543967536No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC76961.0246155361857303No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTCCCATGG53220.7085504006731362No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG39240.5224261127849279No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC34560.46011841126012004No Hit
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG32950.43868349684667113No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCTCC19370.25788465353323275No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATG17540.23352074460366046No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA17210.22912725282947527No Hit
TAGACCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCT14330.19078405189113193No Hit
CGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT12520.1666864151902981No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC11900.15843197609940476No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCTCCCATGGTC11520.15337280375337334No Hit
ACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG11360.15124262592346538No Hit
AAAGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTT10880.14485209243374148No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA9330.1242159947065081No Hit
AGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT9270.1234171780202926No Hit
GGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT7820.10411244143675169No Hit
ACCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT7610.10131658303499749No Hit
TCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG7550.10051776634878201No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG1950.089.16972
ATTGCGG950.089.102171
TAGGGCG750.087.72374
CGTTTTT33950.087.680561
GCTACGA15350.086.641954
CGCTACG15450.086.385343
TCCGCTA15600.085.9131551
CGGGTAT550.085.445175
TAGCGGG4350.085.346952
CTACGAC15750.084.73995
ACGACCA15300.084.467857
AGGGATG20600.084.40825
ATAGCGG1800.083.6020361
GCGTAGG1700.082.987311
CGACCAA15700.082.914468
AGGGAAT15500.082.771555
AAGGGCG2500.082.7109154
AGGGATC7050.082.6575855
AGGGTAC3700.082.558515
AGCGGGA6550.082.5100253
Produced by FastQC (version 0.11.5)