FastQCFastQC Report
Mon 27 Feb 2023
SRR3128585.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128585.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831629
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103591.2456275574805593No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC91641.101933674751602No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG42660.512969124453332No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC37620.4523651772605332No Hit
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG36410.4378154200971827No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTCCCATGG27990.33656834958857856No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCTCC21010.25263669256363114No Hit
TAGACCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCT17400.20922791292751935No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC13650.16413569031382982No Hit
CGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT13540.1628129851171616No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA11610.13960552121198275No Hit
AAAGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTT11280.13563740562197807No Hit
AGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT10770.12950486334651629No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA10490.12613797739136082No Hit
ACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG10320.12409379663287355No Hit
ACCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT8520.1024495297783026No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATG8390.10088633272769469No Hit
TCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG8350.10040534901981533No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACGA17400.090.755034
CGTTTTT39850.089.1500851
CGCTACG17750.088.9654853
TCCGCTA18300.088.452591
CGACCAA17250.087.4574058
TACGACC18450.086.099576
GGACCGT550.085.451167
CTACGAC18600.085.405225
AGGGATG23250.084.899865
ACGACCA18000.084.857757
GACCGAT452.7284841E-1183.552248
AGGGAAT19300.082.551145
GGATGGC17400.082.111697
CCGCTAC19700.082.082622
AGGGTAG3150.082.0602345
ATAGCGG1500.081.5615161
GGGAATG25250.080.780966
CTAACGG353.832247E-880.665241
ACGGGTA700.080.568234
TAGGGTA3050.080.127974
Produced by FastQC (version 0.11.5)