FastQCFastQC Report
Mon 27 Feb 2023
SRR3128584.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128584.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3246445
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT287320.8850296247125702No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC53240.16399476966343185No Hit
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG38990.12010060235118722TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC38700.1192073175427275TruSeq Adapter, Index 20 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG37570.11572658708217758TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT94050.082.012731
CGTAGGG11600.074.584072
AGGGATG56500.073.6960145
AGGGCAT31700.072.3467945
ATAGGGC18650.070.556643
GAGGGAT45650.069.798754
GTAGGGC16200.069.623353
ACGGGTA3650.069.527984
AGTAGGG53350.068.65772
AGTAAGG21800.068.648621
TAAGGGA31250.068.275453
TAGGGCA18000.068.143864
ATAAGGG43200.067.918342
CGAAGGG35350.067.3052
TAGAGGG57300.066.9609762
ATAGGGA32350.066.825513
GTAGGGA26700.066.5333253
TAGGGAT26700.066.5333254
GTAGGGT13100.066.0093153
GGGCGAT14900.065.9202356
Produced by FastQC (version 0.11.5)