FastQCFastQC Report
Mon 27 Feb 2023
SRR3128583.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128583.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3037985
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264550.8708074595496686No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC47550.15651821849021638No Hit
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC44950.14795991421945795TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG42510.13992827482690007TruSeq Adapter, Index 22 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG41620.13699870144190968TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT86100.081.038161
ATAGGGC18100.073.736763
AGGGATG52450.073.562465
ACGGGTA2900.071.302734
CGTAGGG9500.070.738652
GAGGGAT44350.070.1480944
ATAAGGG39700.069.366772
AGGGCAT27950.068.770175
GTACGGG6700.068.73782
GAATAGG19350.068.5215151
TATAGGG22000.067.927992
ATGCGGG15850.067.896992
TAGAGGG54300.067.851672
ATAGGGA28300.067.7513663
GTAGGGA21500.067.5405043
TAGGGCA16700.066.6934744
ATGAGGG44150.066.313462
TAAGGGA28550.066.170473
ATAGCGG7950.065.6469651
AAGAGGG105850.065.5299452
Produced by FastQC (version 0.11.5)