FastQCFastQC Report
Mon 27 Feb 2023
SRR3128582.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128582.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3405482
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328500.9646211608224622No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC57050.16752400981711252No Hit
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC50850.14931807009991538TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG49700.1459411619265643TruSeq Adapter, Index 22 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG46550.13669136997347217TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT100550.082.452641
AGGGATG55400.071.600455
ATAGGGC20500.071.5292743
AGGGCAT32200.071.51935
CGTAGGG12800.071.237182
ATGCGGG16400.071.075972
ATAGCGG7500.069.637381
ACGGGTA3200.069.02894
GAGGGAT46300.069.025734
ATAAGGG45200.068.527042
CTACGAC12700.068.4622345
TAAGGGA34050.067.771553
TATAGGG25800.067.770112
AGGGTAC15300.067.272225
ATAGGGA34400.067.218643
AGTAGGG57650.067.017392
GTAGGGA25000.066.7377243
TAGGGCA19750.066.6306464
AAGGGAT64700.065.376444
TAGCGGG19450.065.246792
Produced by FastQC (version 0.11.5)