FastQCFastQC Report
Mon 27 Feb 2023
SRR3128581.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128581.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2444268
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206760.8458974220502825No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC41640.17035775127768313No Hit
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG30600.12519085468532912No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30210.12359528496875137No Hit
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC27440.11226264877664807Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG26250.10739411553888525No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT70750.082.8081741
CGTAGGG7400.076.250372
ATAGGGA24400.075.118183
TAGGGCA12950.073.6714
TATAGGG17150.073.2047962
AGGGATG44650.073.047995
CGAAGGG25250.072.254142
AGTAGGG39150.071.34232
GAGGGAT34350.071.008594
GAATAGG14450.070.667911
TAAGCGG5350.070.366591
GACCGAT1950.069.8938
GGTAAGG14350.069.192791
AAGAGGG80150.067.935612
AGTAAGG14050.067.655851
ATAGAGG18300.067.629321
AAGGGAC28200.067.3290564
GTAGGGC11700.067.081213
ATGAGGG40000.066.887462
AGGGAAT28950.066.7211465
Produced by FastQC (version 0.11.5)