FastQCFastQC Report
Mon 27 Feb 2023
SRR3128580.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128580.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2349302
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190430.8105811853903842No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC39630.16868840191682466No Hit
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG34740.1478737088718266No Hit
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC32720.13927541031336116Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG29930.12739954250241137No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28340.1206315748252034No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT65850.081.9241
ATAGGGA22750.076.233053
ATAGCGG3650.073.448941
TAGGGCA10700.073.353664
TAAGCGG4800.072.5093461
CGTAGGG6250.072.1920852
GACCGAT2350.071.99098
AGGGTAT14150.070.7461855
AGGGATG42250.070.524895
TATAGGG15050.070.265862
AGTAGGG38050.070.160392
CGAAGGG22750.069.415462
GTAGGGA19950.069.263243
TAGGGTA8650.069.0043954
TAGGGAT17800.068.65034
GAGGGAT32600.068.3360444
GTAGGGC9500.068.273763
AGGGAAT28550.067.986675
ATAGAGG17850.067.98061
TAAGGGA22300.067.233263
Produced by FastQC (version 0.11.5)