FastQCFastQC Report
Mon 27 Feb 2023
SRR3128579.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128579.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2628268
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235550.8962175851168908No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC47220.1796620435967717No Hit
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG38240.14549505605973212No Hit
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC33220.12639502516486142Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG32680.12434044016820202No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30930.11768206286421323No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75550.083.2142261
ATAGCGG4950.073.2000051
AGGGATG46100.073.096775
TAAGGGA27450.072.765663
AGTAGGG42050.071.999922
ATAGGGA27550.070.625033
TAGGGCA13250.070.585764
CGTAGGG7150.069.039152
GAATAGG15100.068.5600741
GTAGGGC12500.068.053193
AGGGAAT31400.067.9526145
ATAAGGG39050.067.53892
AGAGGGC39200.067.3798753
CGAAGGG24500.067.352422
AAGAGGG92900.067.3051152
GAGGGAT36800.066.921154
AAGGGAT54050.066.8668064
AAGGGAC29750.066.8241354
TAAGCGG5000.066.8211441
TATAGGG19200.066.600852
Produced by FastQC (version 0.11.5)