FastQCFastQC Report
Mon 27 Feb 2023
SRR3128578.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128578.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2998476
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295750.986334391204065No Hit
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC36040.12019439208451226TruSeq Adapter, Index 23 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG35470.11829342639394147Illumina PCR Primer Index 7 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG33410.111423269687668Illumina PCR Primer Index 7 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT95550.083.0940251
CGTAGGG8300.072.503772
TAGGGCA16300.071.500934
ATAGGGC17900.070.885663
AGTAGGG49200.070.6168062
ATAGCGG6000.069.811041
AGGGCAT24650.069.014335
ACGGGAT8300.068.510154
TAGCGGG14450.067.9997252
AGGGATG45950.067.807175
ACGGGTA3050.067.795434
GGTAAGG19550.067.646441
TATAGGG22850.067.486412
AGGGAAT33350.067.2156145
TAGAGGG54850.067.199832
AAGGGTA24250.066.8582464
GAATAGG18450.066.577811
TAAGGGA29750.066.345273
TAGGGTA9650.066.230684
AGTAAGG18450.065.557461
Produced by FastQC (version 0.11.5)