FastQCFastQC Report
Mon 27 Feb 2023
SRR3128577.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128577.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2820357
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT275630.9772876270628151No Hit
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC40440.1433861032486313TruSeq Adapter, Index 23 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG38410.13618843288278754Illumina PCR Primer Index 7 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG37720.13374193408848598Illumina PCR Primer Index 7 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT88050.082.474881
ACGGGTA3850.078.118394
TAGGGCA14350.074.6649864
ATAGGGC16700.073.162913
ATAGCGG5450.071.628421
AGTAGGG47300.070.936722
GAATAGG18150.070.744031
GTACGGG6400.069.755372
AGGGCAT23800.068.912485
CGTAGGG7400.068.584522
ATAGGGA26500.067.918323
AAGGGTA19700.067.507844
TATAGGG21300.067.290582
ATGAGGG47700.066.795222
TAGAGGG52700.065.897342
TAGCGGG13850.065.484962
TAAGGGA28150.065.4397663
AGGGTAT18300.065.22775
GTAGGGC12300.064.949813
TAGGGTA8900.064.945514
Produced by FastQC (version 0.11.5)