FastQCFastQC Report
Mon 27 Feb 2023
SRR3128576.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128576.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3148010
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT335561.0659432466860015No Hit
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC46880.14891947611348122TruSeq Adapter, Index 23 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG43460.13805546996356427Illumina PCR Primer Index 7 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG40470.12855740610735036Illumina PCR Primer Index 7 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT101700.085.316531
ACGGGTA3500.077.882954
ATAGGGC18150.073.7990343
TAGGGCA16850.072.5196844
GACCGAT2950.071.692378
ATAGCGG6800.070.580451
GGTAAGG19050.070.148211
ACGGGAT9250.070.116434
TATAGGG26500.069.353522
CGTAGGG8950.069.324782
TAGCGGG17450.068.95762
TAGAGGG57800.068.717362
AGGGCAT25650.068.344555
TAAGGGA30500.067.646783
AGTAGGG52850.067.326892
GAATAGG19800.067.2534261
AGGGTAC15450.067.227395
GAGGGAT38000.066.910784
GTAGGGC14850.066.778783
AGGGAAT36150.066.6947255
Produced by FastQC (version 0.11.5)