FastQCFastQC Report
Mon 27 Feb 2023
SRR3128575.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128575.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2538120
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216590.8533481474477171No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG35240.13884292310844246No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT33050.13021448946464312No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG31100.12253163759002726No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC29010.11429719635005436No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC25620.10094085386033758No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71550.081.125641
CGTAGGG7350.074.856952
TAGGGCA14400.072.77694
ATAACGG2350.072.117281
AGGGCAT23100.071.4079065
ATAGGGA23300.070.593273
GTAGGGT12050.070.589923
TAGGGTA8850.069.563154
GTAGGGC12600.068.627543
AAGGGAC25450.068.507374
ATAGGGC14350.068.44563
AGGGATG32350.068.2770545
ATAGAGG19300.068.053681
TAAGGGA20250.067.765563
TAGAGGG46850.067.7528842
GAATAGG15300.067.691781
AGGGAAT28400.067.6793445
TATAGGG19500.067.523832
AGAGGGC32200.067.4275363
AGTAGGG43350.067.1483152
Produced by FastQC (version 0.11.5)