FastQCFastQC Report
Mon 27 Feb 2023
SRR3128574.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128574.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2416805
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196470.8129327769513883No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG41490.17167293182528173No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG36770.152143015261885No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC32700.1353025999201425No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30090.12450321809165407No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT66750.079.548121
CGTAGGG8300.074.174882
GTACGGG4800.072.452622
AGTAGGG36450.070.7845462
ATAGGGA23550.070.444563
GTAGGGA18750.070.4317863
GTAGGGC10850.070.169583
AGGGACT36950.069.699525
GGTAAGG12800.069.4446951
AAGGGAC26600.069.25774
AGGGATG30550.068.763815
ATAAGGG30600.068.651452
TAGGGCA13700.068.6077354
ATAGGGC13950.068.388883
AAGGGTA14900.068.128864
TAAGGGA20750.067.946463
AGGGAAT25600.067.190025
TAGGGAC13850.066.846724
GATAGGG32850.066.524442
GAATAGG15150.066.4336851
Produced by FastQC (version 0.11.5)