FastQCFastQC Report
Mon 27 Feb 2023
SRR3128573.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128573.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2714298
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241200.8886275567384274No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG47310.17429921106672885No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG41400.1525256254103271No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC37290.13738358868480913No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34220.12607311356380177No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27570.10157322445803667No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT78650.081.730191
CGTAGGG7850.076.644752
GTAGGGC12850.071.319723
ATAGGGA26600.071.026843
GTAGGGA21100.070.385423
AGGGATG34200.069.809695
TGTAGGG18750.069.692442
GAATAGG16650.069.244031
TAGAGGG48350.069.219052
ATAAGGG36650.069.000212
AGTAGGG45350.068.926542
TAGGGCA16050.068.8130654
AAGGGAC28150.068.785514
AGGGAAT26950.068.709315
ATAGGGC16500.068.645373
GGATAGG17800.068.4715651
GTAGGGT10150.068.0658653
GTACGGG6450.067.7743452
ATAACGG2850.067.697011
ATAGAGG19550.067.397191
Produced by FastQC (version 0.11.5)