FastQCFastQC Report
Mon 27 Feb 2023
SRR3128572.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128572.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2842948
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234140.8235817186948196No Hit
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG29780.10475042104181997No Hit
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG29750.10464489677616334No Hit
CTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC29260.10292133377043829No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT84200.081.716571
ATAGCGG6900.077.75541
AGGGATG43500.070.2243045
TAGGGCA14100.069.327824
GGTAAGG18250.068.595181
AGGGTAC12100.068.35825
AGTAGGG44600.068.216992
CGAAGGG27900.067.755562
ATAGGGC16550.067.299783
ACGGGTA2450.067.137514
AAGGGAT47750.067.025044
TAAGGGA30550.066.9177553
ATAGGGA27350.066.842953
GTAAGGG42850.066.6133042
GAATAGG17050.066.24631
AAGAGGG94600.066.0130842
TAGAGGG49450.065.4252242
GTAGGGA21700.065.404943
AAGGGAC28650.065.286264
AAGGGTA20150.065.071754
Produced by FastQC (version 0.11.5)