FastQCFastQC Report
Mon 27 Feb 2023
SRR3128571.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128571.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2631542
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217550.8267016068905607No Hit
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG35090.13334387214796495No Hit
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG34520.13117784173689798No Hit
CTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC33850.1286318059905561No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74350.082.354211
CGAAGGG24800.070.490952
TAGGGCA14350.070.088264
ATAGGGC15800.069.301233
GTAGGGC12600.067.880173
TAGGGTA8050.067.724454
GTAGGGA20750.067.7166063
AGGGATG39750.067.514785
TAAGGGA25200.067.50723
AGGGTAC11000.067.082135
ACGGGTA2600.066.8823854
GGTAAGG15900.066.2531741
ATAGCGG6250.066.215321
GTAAGGG39100.066.104042
ATAGGGA25100.066.091123
AGTAGGG44200.066.025462
TGAAGGG41350.065.9165652
AGGGAAT29100.065.897225
AAGAGGG82100.065.82592
AGTAAGG17450.065.758241
Produced by FastQC (version 0.11.5)