FastQCFastQC Report
Mon 27 Feb 2023
SRR3128570.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128570.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2957791
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267450.9042221035901455No Hit
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG40390.13655461119463816No Hit
CTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC39410.13324132773410968No Hit
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG37550.12695285096208622No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29970.10132561766534552No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT93450.083.179421
ATAGCGG6300.072.446321
ACGGGTA2800.072.1753164
ATAGGGC19000.070.991533
ATAGGGA28650.068.8974153
TAGGGCA15300.068.807354
AGGGATG42800.068.630075
GAATAGG16600.068.028091
AAGAGGG92800.067.977242
TAAGGGA30450.067.75723
CGAAGGG27500.067.00582
AGTAGGG49300.066.743682
AGGGAAT35850.066.7278065
AAGGGAT52900.066.365624
GAGGGAT37850.065.809454
TAGAGGG53600.065.598792
AGAGGGC42350.065.03133
AGAGGGA75150.064.477463
AAGGGTA23800.064.3752444
GTACGGG6500.064.3629152
Produced by FastQC (version 0.11.5)