FastQCFastQC Report
Mon 27 Feb 2023
SRR3128569.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128569.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2582013
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT561032.172839563549835No Hit
CGTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTT46240.17908507819286737No Hit
CGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT44770.17339184581952144No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCT42520.16467771463582873No Hit
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG36800.14252445669328545No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29440.11401956535462834No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT180500.087.8536151
ATAGGGC15900.075.96093
AGGGATG38800.070.7351465
TAGGGCA12400.070.49294
TAGGGCG4700.069.992954
AGTAGGG44100.069.728762
GAGGGAT31750.068.531684
CGTAGGG8600.066.701292
TAGGGTA6400.066.087094
TAAGGGA23800.065.358963
AGTAAGG18550.065.211631
AATAGGG35150.065.144312
AGGGCAT20150.065.0703665
GTAGGGC13300.064.662663
GGTAAGG14600.064.478221
ATAGGGA25000.064.289523
TAGAGGG44450.063.8907552
TAGACGG2950.063.8225021
AGCAGGG47150.063.3234942
ATAGAGG17000.062.8510861
Produced by FastQC (version 0.11.5)