FastQCFastQC Report
Mon 27 Feb 2023
SRR3128568.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128568.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2449786
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT521342.128104250738636No Hit
CGTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTT51430.2099367046754288No Hit
CGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT50100.2045076590363403No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCT46410.18944511887977156No Hit
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG40220.16417760571739737No Hit
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC28680.11707145032259961TruSeq Adapter, Index 13 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25230.10298858757458815No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT169550.087.125111
ATAGGGC14650.073.138483
ACGGGTA2450.070.9715654
AGGGATG39300.070.90915
TAGGGCA12300.070.683064
AGTAGGG36200.069.064012
GTAGGGC11350.068.318263
TAAGGGA20950.067.9684453
AGGGAAT25400.066.790295
TAGGGTC6200.066.702134
CGTAGGG7100.066.189692
ATAGAGG17050.065.9034961
ATAGGGA23150.065.772263
TAGAGGG43050.065.388652
AATAGGG31900.064.967572
GGTAAGG13250.064.933691
GAGGGAT31500.064.897424
ATGAGGG35100.064.801792
GTAGGGT8500.064.68683
TATAGGG18200.064.294922
Produced by FastQC (version 0.11.5)