FastQCFastQC Report
Mon 27 Feb 2023
SRR3128565.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128565.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2365023
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205900.8706046410542307No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28000.11839208329052192No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT68900.080.9361651
ATAGGGC15100.074.072463
ATAGCGG5600.070.5291061
GAATAGG15950.070.160621
TATAGGG19850.068.1849752
AGGGATG38000.068.146425
TAGGGCA12350.067.735914
GGTAAGG14300.067.405441
AGAGGGC35550.066.890993
AGTAGGG41800.066.6707152
AGGGCAT21950.066.588775
ATGCGGG11500.066.202432
AGTAAGG15450.065.735871
AAGAGGG83450.065.6642462
ATAGGGA22000.064.7256853
CGTAGGG7050.064.6605452
GAGGGAT30900.064.487174
TAGAGGG41900.064.380542
GTAGGGC14050.064.221683
AGGGAAT23600.064.123765
Produced by FastQC (version 0.11.5)