FastQCFastQC Report
Mon 27 Feb 2023
SRR3128564.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128564.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2669938
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254700.9539547360275782No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31850.1192911595699975No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT83350.082.4950561
ATAGGGC18050.073.1661453
AGGGATG43050.070.197065
ATAGCGG7000.069.9227751
GAATAGG18200.069.5607451
CGTAGGG6750.068.942292
GGATAGG18850.068.160791
GTAGGGC14550.068.155443
ATAGGGA23750.067.677383
TAGGGCA14450.067.651374
AGGGCAT23000.067.636515
TAGGGCG4950.067.411544
AGTAGGG46450.067.397352
GTAGGGA21300.067.2978743
GTAGGGT10150.067.140263
TAGAGGG45450.066.9151842
AGAGGGC39200.066.5407943
AGGGAAT30750.066.026725
CGAAGGG26950.065.58182
TAAACGG3750.065.261251
Produced by FastQC (version 0.11.5)