FastQCFastQC Report
Mon 27 Feb 2023
SRR3128563.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128563.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2571456
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217470.8457076457851116No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC51300.19949787202269842TruSeq Adapter, Index 21 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG51040.19848677169665746No Hit
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG49480.19242016974041165No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC41030.15955940914408026No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT33480.13019861121481371No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTATAGGAC26400.10266557156723662No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74200.081.6841
AGGGATG47450.074.9768145
ACGGGTA2900.071.305334
AGTAGGG42100.070.468012
TAGCGGG15100.070.3681642
TAGGGCG4150.070.211934
CGAAGGG24300.069.266212
ACGGGAT11500.069.0647054
AGGGCAT23750.069.060415
GTACGGG5900.068.531642
TAGGGCA10450.068.358834
GGATGGC37350.068.072857
GTAGGGC11950.068.0370563
ATAGCGG5700.067.696161
TAAGCGG4950.067.496021
AGTACGG3100.066.7906951
AGGGAAT30250.066.6498645
AAGCGGG31350.066.437132
TAGGGAT20450.066.415864
TAGAGGG43850.066.154632
Produced by FastQC (version 0.11.5)