FastQCFastQC Report
Mon 27 Feb 2023
SRR3128561.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128561.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2710396
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248500.9168401960451535No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC66680.2460157113573072TruSeq Adapter, Index 21 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG62540.23074119058617265No Hit
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG62000.2287488617899377No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC44580.16447781062250683No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34260.12640219362779462No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81100.081.471251
ACGGGTA3300.078.330274
AGGGATG48050.074.825385
GTACGGG5950.072.680162
TAGGGCG4050.071.947814
TAGAGGG46300.070.4568942
GAATAGG16400.070.304051
TAAGGGA24550.070.257943
AGTACGG3500.069.918721
AGTAGGG44500.069.187292
AAGGGAC27550.069.089874
AGGGCAT23050.068.509265
TAGGGAT20200.068.4032754
TAAGCGG6750.068.325131
AGGGAAT35600.068.120935
GGTAAGG14550.067.922621
TAGCGGG15700.067.3642
GAGGGAT44900.067.0953754
CGAAGGG25200.066.404032
AGGGACT40450.065.994955
Produced by FastQC (version 0.11.5)