FastQCFastQC Report
Mon 27 Feb 2023
SRR3128559.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128559.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2290979
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162780.7105259367283593No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC135800.5927596891983733No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG42250.18441897546856606No Hit
CCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG41770.18232380130939657No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC40770.17795885514446008No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC36570.1596260812517269No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCAAT30660.13382924941695232No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAATGACAG29880.13042459140830187No Hit
AGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTA24310.1061118412696057No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA23990.10471505849682602No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTACG23050.084.614063
TAGGGCG3950.084.476534
CGTTTTT66000.084.130121
GCTACGA23200.084.0688254
TCCGCTA23900.082.818951
CGTAGGG5450.082.78282
CTACGAC23750.082.319855
AGGGATG71250.082.253895
CGAAGGG20050.082.038762
ACGACCA23350.081.9157
ACGGGTA3450.081.734814
CTAGCGG2100.080.598831
CGAGGGA16100.079.98163
CGACCAA24350.078.5509348
TAGCGGG10700.077.7418442
AAGGGAT66500.077.0335164
GGATGGC48900.076.884197
AGGGATC24250.076.746455
AGGGCAT27150.076.684715
AGCGGGA21500.076.50593