FastQCFastQC Report
Mon 27 Feb 2023
SRR3128557.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128557.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2977302
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279690.9394075575806552No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC34070.11443246267929823TruSeq Adapter, Index 13 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT33470.11241721531776085No Hit
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG32310.10852107041878856No Hit
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG32050.10764779656212235No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT98600.081.222481
ATAGGGC15900.074.18743
TAGGGCA15200.072.0387044
AGGGATG47300.070.1450655
AGTAGGG46850.069.9524462
ATAGGGA29500.069.935243
TAGAGGG53500.069.69462
TAAGGGA30850.069.312223
GTAGGGA23300.068.980083
ATAAGGG42950.068.53162
TATAGGG23600.068.337932
GAATAGG17550.068.100491
CGTAGGG8150.067.500652
ATAGCGG7250.067.4976961
GACCGAT2300.067.427918
ACGGGAT8350.066.9752044
GAGGGAT37900.066.834874
TAGGGAT17600.066.7545554
AAGGGAT56750.066.083134
GTACGGG6400.065.386752
Produced by FastQC (version 0.11.5)