FastQCFastQC Report
Mon 27 Feb 2023
SRR3128556.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128556.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2806044
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256930.9156306886135784No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC38450.13702564892068692TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG36010.12833013309841185No Hit
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG35910.1279737594991383No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30310.10801683793981848No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT89200.082.599721
GTACGGG6200.074.2847752
TAGGGCA14000.073.515944
ATAGGGC14800.073.035093
AGGGATG41650.072.441165
CGTAGGG8950.071.938772
GGTAAGG16750.071.300631
AGTAGGG45000.070.2858662
GTAGGGA22300.069.546373
CGAAGGG30250.069.290692
GTAGGGC13500.069.2763063
ACGGGAT9900.068.8332444
GAATAGG15500.068.253551
TAAGGGA27650.067.9876863
TAGAGGG48650.067.620852
AAGGGAT47800.067.4468464
ATAGGGA23750.067.279193
TGTAGGG19750.067.103842
GAGGGAT35700.067.0061954
ATAAGGG38900.066.930732
Produced by FastQC (version 0.11.5)