FastQCFastQC Report
Mon 27 Feb 2023
SRR3128555.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128555.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3128959
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT315481.008258657272275No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC44190.1412290797035052TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG43380.13864035930160798No Hit
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG38880.12425857929106773No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34010.10869429736854973No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT106000.081.631241
ATAGGGC15750.074.6002043
ATAGCGG6200.074.372931
GAATAGG17000.073.06941
AGGGATG48700.072.089545
AGTAGGG50650.071.177892
ATAGGGA31700.070.126553
TAGAGGG56450.069.6932452
GTAGGGA23950.069.66323
GAGGGAT36950.068.939074
GTACGGG7250.068.722192
CGTAGGG8800.067.8344352
TAAGGGA34000.067.73263
AGGGAAT33400.067.682935
ATAAGGG42450.067.543152
TAGGGCA15300.067.2718354
TATAGGG24900.066.4465562
GGTAAGG18650.066.352531
ATAGAGG21100.066.229971
GTAAGGG48600.066.1529162
Produced by FastQC (version 0.11.5)