FastQCFastQC Report
Mon 27 Feb 2023
SRR3128554.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128554.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2154636
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT485202.2518884860366204No Hit
CGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT42010.1949749284797989No Hit
CGTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTT36720.17042321765718202No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG33650.1561748713007673No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCT32360.15018778113797412No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC23430.1087422655149176No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT155800.087.949981
ATAGGGC14000.078.217333
TAGGGCA11750.076.795984
GTAGGGC11150.072.498453
AGGGATG34350.072.103945
ATAGGGA20900.071.9579543
AGTAGGG31100.071.5001452
ACGGGTA2450.070.9758764
GTACGGG6500.068.7094352
TATAGGG19250.068.380692
AGATAGG13450.067.895411
TAGAGGG37400.067.375092
TGTAGGG17000.066.922572
AGAGGGC34000.066.902383
AGGGAAT23200.066.849955
TAGGGCG3950.066.6294254
AGGGCAT16150.066.3494645
ATAGAGG17100.065.239891
AGTACGG4200.065.0039441
GATAGGG32200.064.969612
Produced by FastQC (version 0.11.5)