FastQCFastQC Report
Mon 27 Feb 2023
SRR3128553.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128553.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2028893
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT447122.2037633330096758No Hit
CGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT45340.22347161728095075No Hit
CGTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTT42070.2073544538819938No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG38080.18768855725757838No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCT35770.17630303815923265No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC26170.12898659515312044No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT143150.087.149971
TAGGGCG4050.074.260384
AGGGATG29600.073.190075
ATAGGGC11900.073.05443
CGTAGGG6450.072.126872
ATAGGGA19000.071.476913
ACGGGTA1850.071.124394
AGTAGGG29600.070.646372
AGGGAAT21300.070.160145
GTAGGGC11250.069.338933
TAGGGCA9850.067.269034
TAACGGG5950.067.1310652
GAATAGG11450.066.532051
AAGGGCA28750.066.5256044
TAGAGGG35900.066.364392
AGGGACT19650.066.2460865
TAAGGGA17100.066.2281343
GGTAAGG11650.066.197141
TATAGGG17150.066.035052
GTACGGG5450.065.529782
Produced by FastQC (version 0.11.5)