FastQCFastQC Report
Mon 27 Feb 2023
SRR3128552.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128552.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2268234
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT542872.3933597679957184No Hit
CGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT48450.21360230029176885No Hit
CGTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTT44660.1968932658623405No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG44240.19504160505485765No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCT40270.17753900170793666No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC30660.13517123894624627No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT163650.088.043651
ATAGGGC14800.073.673313
TAGGGCA12550.071.527584
ATAGGGA21750.070.65983
AGGGATG34350.070.600355
AGGGCAT17500.069.826335
AGTAGGG33700.069.0412
TATAGGG16250.068.84232
GTACGGG7050.068.6720352
AGAGGGC34000.066.4890443
ATAAGGG26550.066.3893662
TAGAGGG37900.065.978772
CGTAGGG7000.065.805142
ATAGCGG5750.065.4469151
GTAGGGC11850.065.440933
AAGAGGG72050.065.107092
GATAGGG35650.064.7371752
AGATAGG15100.064.173741
GAGGGAT31150.064.123144
AGGGACT25750.063.8814435
Produced by FastQC (version 0.11.5)