FastQCFastQC Report
Mon 27 Feb 2023
SRR3128551.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128551.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649369
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67231.0353127420619093No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTTTCCGAA55290.8514419382508249No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG36730.5656260154088046No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC35060.539908742178946No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC31010.47754050470533704No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG29030.447049366384906No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATG18350.2825820142322778No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA17810.27426624923579657No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCTTTCCGAATC11770.18125287779367355No Hit
CGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT11420.17586303011076906No Hit
ACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG10040.1546116306753171No Hit
AAAGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT8990.13844208762660368No Hit
AGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT7510.11565073171032186No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCTTT7380.11364878828524305No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG6990.10764295801000663No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG6970.10733496671384067No Hit
GGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT6800.10471704069642991No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC6590.10148313208668723No Hit
GAACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT6540.1007131538462723No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCTTTCCGAATCG6520.10040516255010634No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG353.5652192E-1094.087411
TCCGCTA6250.088.065811
CGCTACG6200.087.9291463
GCTACGA6250.087.2257164
CGTTTTT33350.087.1754461
GTAGCGG1000.084.6786651
CGTAGGG1300.083.173472
AGGGATG14950.082.04765
TAGGGTA2300.081.733244
CTAACGG750.081.542421
TAGGGCG1450.081.028654
TACGACC6750.080.764556
AGGGTAT3800.080.388955
AGGGAAT15200.080.079765
AAGGGCA8500.079.617794
CGGGTAT650.079.532735
GGTAAGG3750.079.0334241
AGGGCGA2150.078.6925
ATAGCGG1200.078.406171
CTACGAC7000.077.8801045
Produced by FastQC (version 0.11.5)