FastQCFastQC Report
Mon 27 Feb 2023
SRR3128550.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128550.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631733
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTTTCCGAA71821.136872697801128No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62220.9849097640933748No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG41280.6534406149433384No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC36670.5804667478190945No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG32480.5141412590445646No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC32100.5081260595852995No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATG23630.3740504295327298No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA20560.3254539496907713No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCTTTCCGAATC15190.24044968364799685No Hit
ACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG12400.1962854560391811No Hit
CGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT12300.19470250881305867No Hit
AAAGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT10160.16082743817403872No Hit
ATCCGTACACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGC9230.14610602897110014No Hit
AGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT9210.14578943952587564No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGT8600.13613346144652885No Hit
GGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT7390.11697980001044744No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG7340.11618832639738623No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCTTT7310.1157134422295495No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCTTTCCGAATCG7030.11128118999640671No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG7010.11096460055118222No Hit
GAACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT6970.11033142166073326No Hit
AGAGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT6880.10890676915722307No Hit
ACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT6630.10494940109191701No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTA156.901825E-493.996368
CGTTTTT28450.086.775841
TCCGCTA5600.084.811571
CGCTACG5700.083.270883
GTAGCGG1250.082.762651
ACGACCA5350.082.576257
ATAGCGG800.082.292411
CGTAGGG1550.081.861312
GCTACGA5800.081.841664
GGTAAGG4250.080.771041
TCCGTAA353.8649887E-880.561932
AAGGGCA8000.080.484394
AGGGCGA2750.080.330525
GGATGGC10350.079.919617
AGGGATT7600.079.779545
CGGGAAT2900.079.4175
ACGGGAA2550.079.251834
GGGAATG18750.079.2075966
AGGGATG11300.079.029755
AGGGCGC1250.078.9631965
Produced by FastQC (version 0.11.5)