FastQCFastQC Report
Mon 27 Feb 2023
SRR3128549.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128549.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687258
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78041.1355269782236075No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG45390.6604506604506605No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC40550.5900258709247473No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTTTCCGAA38630.5620887643359553No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36760.5348791865645799No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG34570.503013424361739No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA12630.18377377927939725No Hit
CGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT12470.1814456870636646No Hit
AAAGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT11210.16311196086476987No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATG10610.15438161505577236No Hit
ACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG10300.14987093638779034No Hit
AGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT9640.1402675559978931No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG8380.12193382979899833No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCTTT8250.12004225487371556No Hit
GGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT7760.11291247246303425No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCTTTCCGAATC7710.11218494364561782No Hit
GAACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT7690.11189393211865123No Hit
ACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT7400.10767426497763576No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG7180.10447313818100336No Hit
AGAGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT7100.10330909207313703No Hit
GAATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT6930.10083549409392106No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGG900.088.859421
CGTAGGG1650.088.307072
CGTTTTT36800.086.288511
GCTACGA6900.085.1426244
AGGGCGT950.084.102985
TCCGCTA7000.084.005751
CGCTACG7000.083.92633
AGGGATG14850.082.920315
AGGGAAT14550.081.722875
GGGCGAT1500.081.464466
TACGACC7300.080.477276
ACGACCA7150.080.1936347
GTAGCGG1000.079.973471
GGGAATG18950.079.8606346
TAGCGGG2650.079.8149642
GGAATGC7250.079.735777
TAAGGGA7850.079.628423
GCGTAGG650.079.61161
CTAGCGG650.079.61161
AAGGGCA8650.079.327334
Produced by FastQC (version 0.11.5)