FastQCFastQC Report
Mon 27 Feb 2023
SRR3128548.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128548.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314651
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128820.9798798312251692No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC35160.2674474061937351No Hit
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG32950.2506368610376442No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG31510.2396833836508701No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGGATACGT28810.21914561355066858No Hit
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC28610.21762429724694995No Hit
GGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAA23440.17829827079582336No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT16020.12185743592786223No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG2550.090.347762
CGTTTTT46500.081.9641951
CGAAGGG12650.079.168162
AGGGATG27350.077.499515
AAGGGAT26500.076.260984
AGGGAAT20400.076.0262455
CTACGTA255.560823E-575.196879
GTGTCGA255.560823E-575.196876
TAGACGG1450.074.636611
AGGGATT16450.074.568325
TAGGGCA6700.074.355114
TAAGGGA14300.072.961993
GGTAAGG7500.072.776111
CTACGAC5450.072.437355
AGTAGGG17900.071.708572
AGGGTAG5750.071.110085
GAGGGAT19900.071.087494
CGCTACG5700.070.9093253
ATAGGGC9400.070.4970553
TAGAGGG19850.070.112052
Produced by FastQC (version 0.11.5)