FastQCFastQC Report
Mon 27 Feb 2023
SRR3128547.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128547.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1254195
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115850.9237000625899482No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGGATACGT39190.3124713461622794No Hit
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG38630.3080063307539896No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG35090.2797810547801578No Hit
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC33160.2643926981051591No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC32680.2605655420409107No Hit
GGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAA19360.1543619612580181No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT15130.12063514844182922No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA12650.10086150877654593No Hit
AGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCT12570.10022364943250452No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT42150.081.64831
GACCGAT1050.080.561288
TACGGGT353.868263E-880.561283
AGGGATG23500.080.1898965
TAGGGCA6150.077.94144
AGGGAAT18000.077.279155
CGTAGGG2500.077.070282
ATAACGG700.073.880231
CGAAGGG11400.073.788952
CGCTACG6550.073.1816253
CTACGAC6450.072.859045
AATGCGG3700.072.428051
GAGGGAT19550.072.113684
AAGGGAT23600.071.4867554
CCGTACA16250.071.4313
GGTAAGG7250.071.332651
GGATGGC16100.070.637067
GTAGGGA12650.070.583993
TAAGGGA12400.070.491123
ATGCGGG7150.070.3268052