FastQCFastQC Report
Mon 27 Feb 2023
SRR3128546.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128546.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1401872
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147651.0532345321113483No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG42410.30252405355125145No Hit
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG42350.30209605441866305No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC38490.2745614435554744No Hit
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC37330.26628679365876484No Hit
GGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAA24970.17811897234554938No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGGATACGT19360.13810105344853166No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT16760.11955442436969994No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT51450.083.123121
TAGACGG1650.082.690841
CTACGAC8000.079.308955
AGGGAAT22100.077.4082955
AGGGATG28100.076.936065
TAAGGGA12700.074.752563
CGAAGGG13450.073.392712
TCCGCTA8950.072.543651
CGCTACG8950.071.940913
TACGACC9050.071.145986
TAGGGCA7100.070.827814
GGATGGC18400.070.2414257
GAGGGAT20550.070.210974
TAGGGCG2350.069.9968644
ATAGGGC8100.069.626513
ACGACCA9250.069.60777
TAGGGTC4000.069.32194
AGTAGGG22550.068.998122
GTAAGGG18750.068.942542
GCTACGA9250.068.591524