FastQCFastQC Report
Mon 27 Feb 2023
SRR3128545.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128545.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences711629
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61030.8576097938673101No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC40380.5674305010054397No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTCAATCC26510.37252557161105015No Hit
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG19960.2804832293231445No Hit
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG16820.23635911408894242No Hit
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC15620.21949639489115816TruSeq Adapter, Index 22 (95% over 21bp)
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA10040.14108475062146147No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC9340.131248164422754No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATG8420.11832007970445275No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA7960.11185603734530211No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCGTC7220.10145736050666851No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATCGA254.9220034E-793.991228
TAGGGCG1150.089.904644
CGTAGGG2300.087.910772
CGTTTTT27400.086.069021
TAGGGTC1150.085.818064
AGGGATT11300.084.425745
TCCGCTA6750.083.682991
CGCTACG6750.083.5477453
GGCGATC452.7284841E-1183.5477457
AGGGTAC3600.082.242315
GCTACGA7000.081.906634
CGAGGGA5800.081.837183
GGGCGAT2450.080.56396
AAGGGAT21400.080.375664
AGGGAAT11600.080.2166445
AGGGATG23150.079.983895
GTAGGGC2850.079.15053
ACGACCA7050.078.6593257
TACGACC7350.078.006326
AACGAGG3400.077.529821
Produced by FastQC (version 0.11.5)