FastQCFastQC Report
Mon 27 Feb 2023
SRR3128544.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128544.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences667955
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55770.8349364852422693No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC37530.5618641974384502No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTCAATCC36020.5392578841389016No Hit
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG21450.3211294174008728No Hit
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG18800.2814560861135855No Hit
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC18140.2715751809627894TruSeq Adapter, Index 22 (95% over 21bp)
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA12030.1801019529758741No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATG11700.17516150040047607No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC8360.12515813191008376No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCGTC7720.11557664812749363No Hit
AAAGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTT6940.10389921476746188No Hit
CGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCT6930.10374950408335891No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCGTCAATCCTC6760.1012044224536084No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA6680.10000673698078463No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC156.90264E-493.994167
CGTAGGG2050.091.6947562
ATAGCGG950.089.093791
TAGGGCG600.086.1613164
CGTTTTT23800.085.350351
CGCTACG7800.084.347413
GCTACGA7750.084.291544
TCCGCTA7950.083.988491
GTAGGGC2750.083.7339863
TAAGCGG1750.083.295621
AGGGATG20300.082.187025
CTACGAC7950.082.1715
ACGGGTG1550.081.865884
AAGGGAT19350.081.1215744
ACGACCA7750.080.653057
GTAGGGT1700.080.165493
AAGCGGG8850.079.119112
AGGGATT9950.078.4071965
GAGGGAT14150.078.051694
TACGACC8250.078.043646
Produced by FastQC (version 0.11.5)