FastQCFastQC Report
Mon 27 Feb 2023
SRR3128543.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128543.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences750524
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70420.9382777899174444No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC43470.5791953355255794No Hit
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG24690.3289701595152187No Hit
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG20960.2792715489444708No Hit
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC19520.26008495397882014TruSeq Adapter, Index 22 (95% over 21bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTCAATCC19270.2567539479083947No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC9120.12151510144912088No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCGTC8490.11312096615164872No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA7990.1064589540107978No Hit
AAAGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTT7710.10272822721192128No Hit
CGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCT7690.10246174672628723No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGG301.3145836E-894.109061
CGCTACG8300.086.635053
CGTTTTT31850.086.131211
CGTAGGG2600.084.963762
GCTACGA8500.084.596584
AGGGATG24100.083.0754855
TCCGCTA8850.081.8801961
ACGACCA8400.081.687187
CTACGAC8850.081.250955
CGAAGGG7350.080.573542
ATAGCGG1000.079.99271
AGCGGGA5250.078.777773
GGATGGC16450.078.568257
TAGGGCA4550.078.502334
AGGGCAA6800.078.0997855
CGAGGGA5300.078.034583
AAGGGAT24400.078.009144
CAGGGAT7100.077.447584
GTAGGGC3900.077.125093
AAGGGCA11850.076.942044