Basic Statistics
Measure | Value |
---|---|
Filename | SRR3128543.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 750524 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 7042 | 0.9382777899174444 | No Hit |
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC | 4347 | 0.5791953355255794 | No Hit |
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG | 2469 | 0.3289701595152187 | No Hit |
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG | 2096 | 0.2792715489444708 | No Hit |
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC | 1952 | 0.26008495397882014 | TruSeq Adapter, Index 22 (95% over 21bp) |
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTCAATCC | 1927 | 0.2567539479083947 | No Hit |
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC | 912 | 0.12151510144912088 | No Hit |
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCGTC | 849 | 0.11312096615164872 | No Hit |
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA | 799 | 0.1064589540107978 | No Hit |
AAAGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTT | 771 | 0.10272822721192128 | No Hit |
CGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCT | 769 | 0.10246174672628723 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ACGACGG | 30 | 1.3145836E-8 | 94.10906 | 1 |
CGCTACG | 830 | 0.0 | 86.63505 | 3 |
CGTTTTT | 3185 | 0.0 | 86.13121 | 1 |
CGTAGGG | 260 | 0.0 | 84.96376 | 2 |
GCTACGA | 850 | 0.0 | 84.59658 | 4 |
AGGGATG | 2410 | 0.0 | 83.075485 | 5 |
TCCGCTA | 885 | 0.0 | 81.880196 | 1 |
ACGACCA | 840 | 0.0 | 81.68718 | 7 |
CTACGAC | 885 | 0.0 | 81.25095 | 5 |
CGAAGGG | 735 | 0.0 | 80.57354 | 2 |
ATAGCGG | 100 | 0.0 | 79.9927 | 1 |
AGCGGGA | 525 | 0.0 | 78.77777 | 3 |
GGATGGC | 1645 | 0.0 | 78.56825 | 7 |
TAGGGCA | 455 | 0.0 | 78.50233 | 4 |
AGGGCAA | 680 | 0.0 | 78.099785 | 5 |
CGAGGGA | 530 | 0.0 | 78.03458 | 3 |
AAGGGAT | 2440 | 0.0 | 78.00914 | 4 |
CAGGGAT | 710 | 0.0 | 77.44758 | 4 |
GTAGGGC | 390 | 0.0 | 77.12509 | 3 |
AAGGGCA | 1185 | 0.0 | 76.94204 | 4 |