FastQCFastQC Report
Mon 27 Feb 2023
SRR3128542.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128542.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1922737
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158320.8234095458713283No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG32010.16648142725708195TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG30060.1563396345938108TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC25680.13355960799630942TruSeq Adapter, Index 22 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC21600.11233985719315746No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT19890.10344628516536583No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT52900.079.989291
TAGGGCA11200.077.2077264
CGTAGGG6700.074.386712
GTAGGGC8400.071.612973
ATAGGGC11300.071.533753
AGTAGGG30200.071.3054662
ATAGCGG4100.071.1763461
TAAGGGA20100.070.3771063
ATAGGGA18650.070.305023
GTACGGG3450.069.50492
CGAAGGG18650.068.57322
CGGGTAT2950.068.5026555
TAGCGGG9950.068.046182
AGGGCAC15850.067.899595
TAGGGTA7400.067.31864
AAGGGCG9200.066.918234
GTAAGGG27700.066.538132
GAATAGG11750.066.496381
AAGGGTA15500.066.400814
AGGGCAT18050.065.872525
Produced by FastQC (version 0.11.5)