FastQCFastQC Report
Mon 27 Feb 2023
SRR3128540.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128540.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2008607
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176630.8793656499255453No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG40680.20252841894905274TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG38420.1912768401185498TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC33090.16474103694749645TruSeq Adapter, Index 22 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC24050.11973472162548472No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT20980.10445049728493429No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT57750.082.866861
CGTAGGG6750.077.992022
TAGGGCA10350.074.01664
GTAGGGC9800.073.374853
AGTAGGG32800.073.085682
TAAGGGA21450.072.962353
ATAGGGC13400.070.4974063
ATAGCGG3950.070.285681
ATAGGGA19350.068.736493
AAGGGAC21200.068.7238854
AAGGGTA15550.068.306164
TAGGGAT12900.068.129274
ATAACGG1250.067.760161
TATAGGG16450.067.148742
ATAAGGG27500.067.0022352
GAATAGG11450.066.987541
TAGGGTA8000.066.9725344
GGTAAGG12300.066.949121
AGGGTAC9900.066.936935
GTACGGG4500.066.850312
Produced by FastQC (version 0.11.5)