FastQCFastQC Report
Mon 27 Feb 2023
SRR3128539.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128539.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1699236
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159670.9396575872921713No Hit
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG27730.163190987008279No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC26220.15430464043840877No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG26110.1536572906882858No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT19190.11293310640782092No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGG4300.082.0876851
CGTTTTT52000.081.1847151
TAGGGCA7400.075.576474
TAAGGGA16200.075.137393
AGGGATG29850.073.211615
ACGGGTA1500.072.062274
CGGGTAT1250.071.435645
TAGGGCG2900.071.3059844
ATAGGGC10150.070.8429643
TATAGGG13750.070.4579242
GTAGGGA12900.069.949223
AGTAGGG26250.069.5132752
TAGGGTC4400.069.427584
GTACGGG3400.069.160232
ATAGGGA15450.069.05083
TACAGGG10900.069.033342
ACGGGAT5700.068.434424
AAGGGAC17150.067.960874
TAGCGGG9450.067.681892
ATAGAGG12450.067.665751
Produced by FastQC (version 0.11.5)